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rabbit anti mouse mtor  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti mouse mtor
    Rabbit Anti Mouse Mtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 8569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mtor/mTOR+Antibody/us12582614-163-74-78
    Average 97 stars, based on 8569 article reviews
    rabbit anti mouse mtor - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Western Blot:

    Article Title: TBCK-deficiency leads to compartment-specific mRNA and lysosomal trafficking defects in patient-derived neurons
    Article Snippet: Blots were washed 3 times with TBS-T and incubated with secondary antibodies (LI-COR Biosciences) for 2 hours at RT, washed again for 3 times in TBS-T, followed by LI-COR system detection, bands were quantified using Image Studio (LI-COR Biosciences). .. The following antibodies were used for western blot analysis: rabbit anti-LC3B (1:1000, Cell signaling, 27755), rabbit anti-p62 (1:1000, Cell signaling, 51145), mouse anti-mTOR (1:1000, Cell signaling, 45175), rabbit anti-phospho mTOR (1:1000, Cell signaling, 55365), rabbit anti-RAB5 (1:1000, Cell signaling, 35475), rabbit anti-Rab7 (1:1000, Cell signaling, 9367T), rabbit anti-TUJ1 (1:1000, Cell signaling, 5568), rabbit anti-Tom20 (1:1000, Cell signaling, 72610), mouse anti-Flag (1:1000, Sigma, F1804), goat anti-GAPDH (1:2000, RD Systems, AF5718), rabbit anti-TBCK (1:1000, Novus, NBP1-83166), rabbit anti-VDAC (1:1000, Abcam, ab15895), mouse anti-LAMP1 (1:500, DSHB, H4A3), rabbit anti-Cyt B (1:1000, Proteintech, 55090-1-AP), rabbit anti-RAB7 (1:1000, Cell signaling, 9367T), rabbit anti-PPP1R21 (1:1000, Atlas Ab, HPA036791), rabbit anti-C12ORF4 (1:1000, Sigma, HPA037871), rabbit anti-JIP4 (1:1000, Cell signaling, 5519), rabbit anti-TRIM27 (1:1000, IBL, 18791), rabbit anti-CRYZL1 (1:500, Novus, NBP1-89367), OXPHOS cocktail (1:500, Abcam, ab110413), goat anti-rabbit IgG (1:3000, LI-COR, 926-32211), goat anti-mouse IgG (1:3000, LI-COR, 926-68070), donkey anti-goat IgG (1:3000, LI-COR, 926-68074) ..

    Incubation:

    Article Title: Dysregulation of the mTOR-FMRP pathway and synaptic plasticity in an environmental model of ASD
    Article Snippet: Proteins (20 μg) were separated on a 4-15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels (4568086 BioRad, Hercules, California, USA) and blotted on a PVDF membrane (Roche-Merck). .. Membranes were incubated using the following primary antibodies: rabbit anti-FMRP (1:1000 rAMII, [14]) or rabbit anti-FMRP (1:1000, AB17722 Abcam, Cambridge, UK), rabbit anti-p-FMRP (Ser499) (1:500, homemade, see Fig. S5f), mouse anti-mTOR (1:1000, 4517 Cell Signaling Technology), rabbit anti-p-mTOR (Ser2448) (1:500, 2971 Cell Signaling Technology), mouse anti-eIF4E (1:1000, SC-271480 Santa Cruz Biotechnology, Dallas, Texas, USA), rabbit anti-p-eIF4E (Ser209) (1:500, 9741 Cell Signaling Technology), rabbit anti-4E-BP1 (1:1000, 9644 Cell Signaling Technology), rabbit anti-p-4E-BP1 (Thr37/46) (1:500, 2855 Cell Signaling Technology), mouse anti-4E-BP2 (1:500, MABS1865 Sigma-Aldrich – Merck), mouse anti-p-p70 S6 Kinase (p-S6K1) (Thr389) (1:500, 9206 Cell Signaling Technology), rabbit anti-p70 S6 Kinase (S6K1) (1:1000, 9202 Cell Signaling Technology), rabbit anti-p-S6 Ribosomal Protein (p-rpS6) (Ser235/236) (1:500, 2211 Cell Signaling Technology), mouse anti-S6 Ribosomal Protein (rpS6) (1:1000, 2317 Cell Signaling Technology), rabbit antiTuberin/TSC2 (1:1000, 4308 Cell Signaling Technology), mouse anti-Vinculin (1:2000, V9131 Sigma-Aldrich - Merck) and mouse anti-β-Actin (1:5000, A5441 Sigma-Aldrich – Merck, Darmstadt, Germany). .. The following secondary antibodies were used: anti-rabbit or anti-mouse IgG Dylight 800 (1:2500, SA5-35571 and 1:5000 SA5-35521, respectively, Invitrogen - Thermo Fisher Scientific) and anti-mouse or anti-rabbit IgG Dylight 680 (1:1000, 35518 and 35568 Invitrogen - Thermo Fisher Scientific), and anti-rabbit IgG or anti-mouse IgG, HRPlinked Antibody (1:5000, 7074S and 7076S Cell Signaling Technology, Danvers, Massachusetts, USA).

    Immunohistochemistry:

    Article Title: Vitamin D Regulates Olfactory Function via Dual Transcriptional and mTOR-Dependent Translational Control of Synaptic Proteins.
    Article Snippet: Capillary cartridges (Protein Simple, SM-W004-1), anti-rabbit detectionmodule chemiluminescence (Protein Simple DM-001), anti-mouse detection module chemiluminescence (Protein Simple DM-002), and EZ standard pack (Protein Simple PS-ST01EZ-8) were used according to the manufacturer’s instructions. .. The following antibodies were used (if notmentioned in the immunohistochemistry section): mouse anti-β-actin (1:200, Novus, NB600501), rabbit anti-AKT (1:200, Cell Signaling Technology, 4691), rabbit antip-AKT (1:20, Cell Signaling Technology, 4060), rabbit anti-eIF4E (1:100, Cell Signaling Technology, 2067), rabbit anti-eIF4EBP1 (1:100, Cell Signaling Technology, 9644), rabbit anti-p-eIF4EBP1 (1:200, Cell Signaling Technology, 2855), rabbit anti-eIF4EBP2 (1:20, CST, 2845), rabbit anti-GAPDH (1:5000, Cell Signaling Technology, 5174), mouse anti-gephyrin (1:4000, Synaptic Systems, 147 111), rabbit anti-GFP (1:1000; Invitrogen A-11122), rabbit anti-mGluR1 (1:500, Cell Signaling Technology, 12 551), rabbit antiMNK (1:100, Cell Signaling Technology, 2195), rabbit anti-p-MNK (1:20, Cell Signaling Technology, 2111), mouse anti-mTOR (1:1000, CST, 4517), rabbit anti-p-mTOR (1:50, CST, 5536), rabbit anti-PI3K (1:3000, Cell Signaling Technology, 4249), rabbit anti-p-PI3K (1:20, Cell Signaling Technology, 17 366), rabbit anti-PSD95 (1:100, Cell Signaling Technology, 3409), rabbit anti-Rps6 (1:100, Cell Signaling Technology, 2217), rabbit anti-S6K (1:200, CST, 33 475), rabbit anti-p-S6K (1:50, CST, 9234), mouse antisynapsin1 (1:7000, Synaptic Systems, 106 011), mouse anti-VGAT (1:20, Synaptic Systems, 131 011),mouse anti-vGlut1 (1:7000, Synaptic Systems, 135 011), and rabbit anti-vGlut2 (1:100, Cell Signaling Technology, 16 066). .. RNA Library Construction and Sequencing (Bulk RNA-seq): Total RNA of OBs was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen, Hilden, Germany).

    other:

    Article Title: Loss of Cldn5 -and increase in Irf7-in the hippocampus and cerebral cortex of diabetic mice at the early symptomatic stage
    Article Snippet: mouse anti-mTOR, dilution 1:1000 (Cell Signaling 4517S); rabbit anti-mTOR-P Ser2448, dilution 1:1000 (Cell Signaling 5536S); rabbit anti-Calnexin, dilution 1:10000 (Abcam ab22595); mouse anti-β-Actin, dilution 1:10000 (Sigma A5441); rabbit antiClaudin-5, dilution 1:1000 (Cell Signaling 49564); rabbit anti-IRF7, dilution 1:750 (Cell Signaling 72073); mouse anti- IkB dilution 1:1000 (Cell Signaling 4814); rabbit antiHIF-3, dilution 1:750 (NovusBio NBP1-03155); rabbit anti-PER2, dilution 1:750 (abcam 180655).

    Article Title: Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke
    Article Snippet: Mouse anti-mTOR , Cell Signaling , Cat# 9964, RRID: AB_10696892.



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    Image Search Results


    AKT/mTOR pathway inhibition by GEM and SSD combination therapy. (A) Immunocytochemistry staining of AKT/mTOR signaling proteins in MIA PaCa-2 and AsPC-1 cells treated with Ctrl, G0.25, S4 or the combination (G0.25 + S4; magnification, ×40; insets show phosphoprotein details). Scale bar, 50 µm. (B) Western blot analysis of p-AKT (60 kDa), AKT (56 kDa), p-mTOR (289 kDa), mTOR (289 kDa) and β-actin (42 kDa). Semi-quantification of phosphorylation ratios in (C) MIA PaCa-2 and (D) AsPC-1 cells. In the figure, GEM represents 0.25 µmol/l GEM and SSD represents 4 µmol/l SSD. Data are presented as the mean ± SD (n=5). Compared with GEM monotherapy, the G0.25 + S4 combination led to a greater reduction in both p-AKT/AKT and p-mTOR/mTOR ratios (both P adj <0.0001), with large effect sizes (η 2 =0.73 and 0.81, respectively). All P-values are Benjamini-Hochberg-adjusted. ****P<0.0001 for G0.25 + S4 vs. Ctrl; ## P<0.01, ### P<0.001 for G0.25 + S4 vs. G0.25. Statistical analysis was performed using one-way ANOVA with Tukey's post hoc test. Ctrl, control; GEM, gemcitabine; SSD, Saikosaponin D; p-, phosphorylated.

    Journal: Oncology Reports

    Article Title: Saikosaponin D overcomes gemcitabine resistance in pancreatic cancer via AKT/mTOR pathway inhibition and synergistic induction of apoptosis and autophagy

    doi: 10.3892/or.2025.9033

    Figure Lengend Snippet: AKT/mTOR pathway inhibition by GEM and SSD combination therapy. (A) Immunocytochemistry staining of AKT/mTOR signaling proteins in MIA PaCa-2 and AsPC-1 cells treated with Ctrl, G0.25, S4 or the combination (G0.25 + S4; magnification, ×40; insets show phosphoprotein details). Scale bar, 50 µm. (B) Western blot analysis of p-AKT (60 kDa), AKT (56 kDa), p-mTOR (289 kDa), mTOR (289 kDa) and β-actin (42 kDa). Semi-quantification of phosphorylation ratios in (C) MIA PaCa-2 and (D) AsPC-1 cells. In the figure, GEM represents 0.25 µmol/l GEM and SSD represents 4 µmol/l SSD. Data are presented as the mean ± SD (n=5). Compared with GEM monotherapy, the G0.25 + S4 combination led to a greater reduction in both p-AKT/AKT and p-mTOR/mTOR ratios (both P adj <0.0001), with large effect sizes (η 2 =0.73 and 0.81, respectively). All P-values are Benjamini-Hochberg-adjusted. ****P<0.0001 for G0.25 + S4 vs. Ctrl; ## P<0.01, ### P<0.001 for G0.25 + S4 vs. G0.25. Statistical analysis was performed using one-way ANOVA with Tukey's post hoc test. Ctrl, control; GEM, gemcitabine; SSD, Saikosaponin D; p-, phosphorylated.

    Article Snippet: The following antibodies were purchased from Proteintech Group, Inc.: Mouse monoclonal antibodies against mTOR (1:500; cat. no. 66888-1-Ig), phosphorylated (p-)mTOR (Ser2448) (1:2,000; cat. no. 67778-1-Ig), p-AKT (Ser473) (1:500; cat. no. 66444-1-Ig), caspase-3 (1:2,000; cat. no. 66470-2-Ig), cleaved caspase-3 (1:1,000; cat. no. 66470-2-Ig), Bax (1:500; cat. no. 60267-1-Ig), AKT (1:500; cat. no. 60203-2-Ig) and β-actin (1:2,000; cat. no. 66009-1-Ig); as well as rabbit antibodies, including polyclonal antibodies against Bcl-2 (1:1,000; cat. no. 12789-1-AP), Beclin 1 (1:1,000; cat. no. 11306-1-AP) and LC3 (1:1,000; cat. no. 14600-1-AP).

    Techniques: Inhibition, Immunocytochemistry, Staining, Western Blot, Phospho-proteomics, Control

    Proposed mechanism of GEM and SSD combination therapy. Co-treatment with GEM and SSD synergistically inhibits the AKT/mTOR pathway, concurrently inducing apoptosis (via Bax/Bcl-2 modulation and caspase-3 activation) and autophagy (via Beclin 1 activation and LC3 conversion), thereby overcoming GEM resistance in pancreatic cancer cells. GEM, gemcitabine; P, phosphorylated; SSD, Saikosaponin D.

    Journal: Oncology Reports

    Article Title: Saikosaponin D overcomes gemcitabine resistance in pancreatic cancer via AKT/mTOR pathway inhibition and synergistic induction of apoptosis and autophagy

    doi: 10.3892/or.2025.9033

    Figure Lengend Snippet: Proposed mechanism of GEM and SSD combination therapy. Co-treatment with GEM and SSD synergistically inhibits the AKT/mTOR pathway, concurrently inducing apoptosis (via Bax/Bcl-2 modulation and caspase-3 activation) and autophagy (via Beclin 1 activation and LC3 conversion), thereby overcoming GEM resistance in pancreatic cancer cells. GEM, gemcitabine; P, phosphorylated; SSD, Saikosaponin D.

    Article Snippet: The following antibodies were purchased from Proteintech Group, Inc.: Mouse monoclonal antibodies against mTOR (1:500; cat. no. 66888-1-Ig), phosphorylated (p-)mTOR (Ser2448) (1:2,000; cat. no. 67778-1-Ig), p-AKT (Ser473) (1:500; cat. no. 66444-1-Ig), caspase-3 (1:2,000; cat. no. 66470-2-Ig), cleaved caspase-3 (1:1,000; cat. no. 66470-2-Ig), Bax (1:500; cat. no. 60267-1-Ig), AKT (1:500; cat. no. 60203-2-Ig) and β-actin (1:2,000; cat. no. 66009-1-Ig); as well as rabbit antibodies, including polyclonal antibodies against Bcl-2 (1:1,000; cat. no. 12789-1-AP), Beclin 1 (1:1,000; cat. no. 11306-1-AP) and LC3 (1:1,000; cat. no. 14600-1-AP).

    Techniques: Activation Assay